Protein Purification Reagents and Kits
Active Motif Histone Extraction Kit
The Histone Extraction Kit provides a simple method to acid precipitate histones from cell culture, tissue and primary cells (e.g. T-cells) while preserving histone post-translational modifications. The highly basic nature of histone proteins often precludes them from being isolated using traditional methods for protein extraction. Instead, histones must be isolated under acidic conditions to retain solubility. The inclusion of inhibitors helps to preserve histone modifications such as acetylation, methylation and phosphorylation. Each Histone Extraction Kit contains enough reagents for extractions from 2 x 96-well plates (192 rxns), or 100 rxns from cultured cell lines and tissues. Kits include buffers, protease inhibitors, phosphatase inhibitors, deacetylase inhibitors and optimized protocols for efficient recovery of core histones (H2A, H2B, H3 & H4). For more complete information, please visit the Active Motif website.
STEMCELL Technologies EasySep™ PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ PE Positive Selection Kit (Catalog #18557) for even faster cell isolations.
LifeSensors Tandem Ubiquitin Binding Entities (TUBE2) Agarose 1mL
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitin state. Agarose coupled TUBEs allows for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or for identification by western blotting. This product is protected by one or more US or Foreign patents.
LifeSensors Control Agarose Beads (negative control)
Control Agarose Beads. For use in “pre-clearing” cell lysates, as well as a negative control for the pulldown of polyubiquitylated proteins in conjunction with agarose-conjugated TUBEs (UM401 and UM402). Control Agarose is generated by blocking an activated matrix (identical to that used for TUBEs conjugation) with ethanolamine. Control Agarose (200 µl resin) is supplied as a 50% slurry in PBS, pH 7.2, 20% ethanol.
Hampton Research HR2-557/2.0 M Calcium chloride dihydrate - 100 ml
HR2-557/2.0 M Calcium chloride dihydrate - 100 ml. Please note items from this supplier are non-returnable.
Norgen Biotek Corp ProteoSpin™ Urine Protein Concentration Micro Kit
This kit concentrates dilute solutions of proteins from small volumes of urine using a rapid and convenient spin column. This kit simultaneously removes salts while concentrating dilute urine proteins ready for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry and protein microarrays. Since there is no molecular weight cutoff with this spin column all sizes of proteins can be concentrated and desalted. Twelve samples can be processed in 20 minutes. Urine protein analysis can also be used for the identification of potential biomarkers in urine, and to diagnose and/or monitor renal and other disease. This kit is also available in a 96-well format for high throughput applications.
Biotage PROPLUS LX PHYTIP COLUMNS (1ML
ProPlus LX PhyTip columns (1mL volume) containing 80 uL affinity media per column 96/PACK
ILEX LIFE SCIENCES LLC EndoTrap red 50, 50 mL settled resin, Affinity chromatography endotoxin (LPS) removal resin
EndoTrap red endotoxin removal products, manufactured in Germany by LIONEX, are intended for removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts and nucleic acids. EndoTrap red is specially developed for buffers containing calcium chelators and for low-salt conditions.Endotoxin removal with EndoTrap red works effectively in the pH range of 6-9 and NaCl concentrations in the range of 50-250 mM. Buffers such as HEPES, PBS, TRIS, MOPS, MES, PIPES and also Citrate, Acetate, Glycine and Carbonate buffers are recommended. EndoTrap red can be used with chelators of divalent cations (like EDTA). Phosphate buffers with NaCl concentrations below 100 mM are recommended for EndoTrap red.Method: affinity chromatography.Format: pre-filled gravity flow columns or bulk resin.LPS Binding Capacity: 2,000,000 EU/ml.Sample Recovery Rate: > 95%.pH Sample Range 6-9.Non-toxic, no cytotoxic effects.Re-use at least 3 times.
Perkin Elmer US LLC Clairion Conventional Trap Basic Kit, 220 V
Includes: Clairion Basic Pump, 220 V Clairion Transport Case with Foam Insert Clairion CT-Pump Interface CT to Pump Interface O-ring Kit O-Ring/Seal Replacement Kit Seal Removal Tool USB Drive with Pump User Manual
National Diagnostics ProtoGel (30%), 37.5:1 Acrylamide to Bisacrylamide Stabilized Solution, 1 liter
ProtoGel (30%); National Diagnostics; 37.5:1 acrylamide to bisacrylamide stabilized soln.; Optimized for SDS-PAGE of proteins (Laemmli gels); Consistently crystal clear gels, zero fluorescence; Stabilized for long shelf life; Size: 1L
LifeSensors SUMOSTAR PROTEASE 500 UNITS
Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins made with SUMOstar expression systems (Cat.#s: 1100A, 1106, 1100K, 1101, 2100, 2101, 2160, 2161, 3100, 3101, 7110, 7111, 7120, 7121). Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar. As a result, SUMOstar Protease 1 will not cleave within the protein of interest. SUMOstar Protease 1 will not cleave SUMO-tags from proteins expressed with the SUMOpro or SUMOpro3 systems. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1 and to cleave SUMOpro3 tag please use SUMO Protease 2. This product contains 5000 units of SUMOstar Protease, where 1 U will cleave >90 µg of SUMOstar-GFP in 1 hour at 37C
American Research Products Inc Mouse Creatine Kinase MB isoen
Mouse Creatine Kinase MB isoenzyme CK-MB ELISA Kit from CUSABIO Detection Range 1 25 mU/mL-80 mU/mL Sensitivity 0 312 mU/mL Sample serum plasma cell culture supernates tissue homogenates Method Sandwich
Cell Signaling Technology SimpleChIP Human 28S rDNA Repeat Primers 500 µl
SimpleChIP Human 28S rDNA Repeat Primers 500 µl